Rapid glutamic acid decarboxylase test for identification of Bacteroides and Clostridium spp

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Rapid glutamic acid decarboxylase test for identification of Bacteroides and Clostridium spp.

A rapid 4-h test for glutamic acid decarboxylase is described for the identification of certain anaerobic bacteria. The test substrate consisted of 1.0 g of L-glutamic acid, 0.3 ml of Triton X-155, and 0.05 g of bromcresol green sodium salt in 1 liter of water. The substrate was dispensed in 0.5-ml amounts into test tubes, and a turbid suspension was made with the test organism. The test was th...

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A rapid glutamic decarboxylase test for identification of bacteria.

A simple rapid glutamic decarboxylase test is described. This test was found useful in the identification of Escherichia coli, Shigella sp., Providencia alcalifaciens, Clostridium perfringens and Bacteroides fragilis.

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Characterization of anaerobic bacteria by using a commercially available rapid tube test for glutamic acid decarboxylase.

A rapid glutamic acid decarboxylase microdilution test for presumptive identification of certain anaerobic bacteria was marketed recently by Carr-Scarborough Microbiologicals, Inc., Stone Mountain, Ga. The test was evaluated with 474 clinical isolates, representing 11 genera and 54 species, and was found to be a useful aid in the presumptive identification of Bacteroides fragilis, B. distasonis...

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New rapid identification test for Clostridium difficile.

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Conventional methods for detecting ornithine decarboxylase activity require an extended period of incubation. However, with a few simple modifications, accurate results were obtained within a few hours rather than several days. The broth medium was modified, primarily by omitting glucose and by decreasing the pH to 5.5. A 1-ml amount of this broth was inoculated with one colony and then overlai...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1984

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.19.5.592-593.1984